定标线的设置与断面数据的采集量直接关系到三维重建图像的失真度。预置定标线是断面标本进行三维重建的依。定标线决定了重建过程中片间各结构的位置关系。而断面数据的采集量决定了重建结构的精细度,特别是细小结构。 -Calibration line settings and the volume of data collection section is directly related to the three-dimensional reconstruction image distortion. Preset calibration line is a cross-section specimens in accordance with three-dimensional reconstruction. Calibration line determined that the process of rebuilding the structure of inter-chip location. Data collection and cross-section determines the volume of the reconstruction of the fine structure of degrees, especially in small structures.