文件名称:vetioatate
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We propose a novel fluorescence emission difference microscopy (FED) system based on focusing cy-
lindrical vector beams. In conventional FED, a Gaussian beam and a 0–2 π vortex phase plate are used to
generate solid and hollow spots. We focus radially polarized and azimuthally polarized cylindrical vector
beams to obtain an expanded solid spot and a shrunken hollow spot, taking advantage of the optical
properties of cylindrical vector beams to improve the conventional FED performance. Our novel method
enhances FED performance
lindrical vector beams. In conventional FED, a Gaussian beam and a 0–2 π vortex phase plate are used to
generate solid and hollow spots. We focus radially polarized and azimuthally polarized cylindrical vector
beams to obtain an expanded solid spot and a shrunken hollow spot, taking advantage of the optical
properties of cylindrical vector beams to improve the conventional FED performance. Our novel method
enhances FED performance
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1-s2.0-S0030401815004320-main.pdf