The purposed approach utilizes Differential Evolution (DE) to optimize an image processing chain which has successfully been used to segment breast ultrasound and X-ray lung images. The training is based on three sample images provided by an expert. As a case study, for each image modalities (ultrasound and x-ray), six test images are used for performance investigation. Details about the proposed algorithm and also conducted experiments are provided.-The purposed approach utilizes Differential Evolution (DE) to optimize an image processing chain　which has successfully been used to segment breast ultrasound and X-ray lung images. The training is based on three sample images provided by an expert. As a case study, for each image modalities (ultrasound and x-ray), six test images are used for performance investigation. Details about the proposed algorithm and also conducted experiments are provided.